Monoclonal antibodies block cell-cell adhesion in Dictyostelium discoideum.
نویسندگان
چکیده
منابع مشابه
Monoclonal antibodies block cell-cell adhesion in Dictyostelium discoideum.
Of 39 monoclonal antibodies that bind the cell surface of aggregating Dictyostelium discoideum, 4 block 76-98% of cell-cell adhesion measured in an in vitro assay. The active antibodies all bind in the range of 10(6) antigenic sites/cell surface and react with more than one material on nitrocellulose blots prepared after polyacrylamide gel electrophoresis of whole aggregating cells in sodium do...
متن کاملEvidence for another cell-adhesion molecule in Dictyostelium discoideum.
We raised a rabbit antiserum that completely blocked cell-cell adhesion of aggregating Dictyostelium discoideum cells in an in vitro assay. All adhesion-blocking activity of this antiserum was adsorbed with a D. discoideum fraction containing molecules having molecular weights as high as about 10(6), even after the material had been extensively digested with Pronase. The properties of this frac...
متن کاملInter-relation of cell adhesion and differentiation in Dictyostelium discoideum.
During the development of Dictyostelium discoideum single cells aggregate to form a multicellular organism. The change from the single cell state to multicellularity is mediated by chemotactic cell movement and intercellular adhesion. Dictyostelium represents a side branch in the evolution of multicellular organization in eukaryotes. Therefore, the general importance of any work done in Dictyos...
متن کاملCell Substratum Adhesion during Early Development of Dictyostelium discoideum
Vegetative and developed amoebae of Dictyostelium discoideum gain traction and move rapidly on a wide range of substrata without forming focal adhesions. We used two independent assays to quantify cell-substrate adhesion in mutants and in wild-type cells as a function of development. Using a microfluidic device that generates a range of hydrodynamic shear stress, we found that substratum adhesi...
متن کاملSerological Investigations of Cell Adhesion in the Slime Molds, Dictyostelium Discoideum, Dictyostelium Purpureum, and Polysphondylium Violaceum
1. Antibodies to slime molds were produced by injecting D. discoideum and D. purpureum amebas from 48 hour cultures into rabbits. 2. Anti-D. discoideum and anti-D. purpureum sera caused agglutination of homologous amebas from 24 to 26 hour cultures, agglutination of certain heterologous amebas from 30 to 36 hour cultures, and agglutination of all heterologous amebas from 43 to 48 hour cultures....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32477-3